Buffers & Stocks

• 98% formamide loading dye

• Diluting 1kb ladder/dye

• Gelco Fluorescein Ladder

• Proteinase K

• Glassware Cleaner

• dNTPs (Promega)

• 1M Tris-Cl

• 0.5M EDTA (pH 8.0)

• 1X TE (10mM Tris; 1mM EDTA)

• 1X TLE (10mM Tris; 0.1 mM EDTA)

• 10X TNE

• 10XTBE "One-Shots"

• Acrylamide (manual gel)

FujiFilm

• DNA Quantification

ABI 3730xl Maintenance

• General Maintenance

• Proteinase K Capillary Wash

FluorImager

Applied Biosystems 3730xl

Acrylamide gels

• Pouring

• Running

Sequencing

• Excising fragments for sequencing

Fluidigm

• EP1 Protocol

Other

• Tissue Sampling Protocol

• Ordering and Receiving Supplies

• PCR Tips and Tricks

• pH meter

• Microsatellite Library Screening

• Developing Primers using DNASTAR software

• Sephadex Cleanup

Archived Protocols & Procedures

Last Updated:  3/23/2011