Buffers & Stocks

• 98% formamide loading dye

• Diluting 1kb ladder/dye

• Gelco Fluorescein Ladder

• Proteinase K

• Glassware Cleaner

• dNTPs (Promega)

• 1M Tris-Cl

• 0.5M EDTA (pH 8.0)

• 1X TE (10mM Tris; 1mM EDTA)

• 1X TLE (10mM Tris; 0.1 mM EDTA)

• 10X TNE

• 10XTBE "One-Shots"

• Acrylamide (manual gel)

FluorImager

• General Use of Fluorimager

• Converting Image to a .tif File

• DNA Quantification

Acrylamide gels

• pouring

• running

• Making your own Polyacrylamide gel for the BaseStation

• BaseStation Use Protocol

• BaseStation Run Model

FluorImager

BioRad

BaseStation

Sequencing

• Excising fragments for sequencing

• ABI BigDye Terminator cycle sequencing of plasmids on the Basestation

• ABI BigDye Terminator cycle sequencing of PCR products on the BaseStation

• Analyzing BaseStation sequencing gels using Cartographer

AFLP

• AFLP protocol

• Making Adapters

SSCP

• SSCP protocols

• Cutting Bands & Reamplification For Sequencing

   

Other

• Tissue Sampling Protocol

• Ordering and Receiving Supplies

• PCR Tips and Tricks

• pH meter

• Microsatellite Library Screening

• Developing Primers using DNASTAR software

Archived Protocols & Procedures