98% Formamide Loading Dye
for denaturing polyacrylamide gels
amount
Formamide
200 ml
Bromophenol Blue
10 mg
Xylene Cyanol FF
10 mg
EDTA
4 ml of 0.5 M pH 8.0 stock
Transfer into 50 mL conical tubes and store at -20º
When supply in 4º runs low, thaw one tube and add to 4º beaker