DNA Extraction from fish fins

  1. Put 1cm2 of fin tissue to 700ml of TNES-Urea Buffer
  2. Add 3.5 ml of 10mg/ml Proteinase-K
  3. Digest O/N at 37 C
  1. Extract two times with an equal volume of Phenol:Chloroform:Isoamyl Alcohol (25:24:1)
  1. Extract one time with an equal volume of C:I (24:1)
  2. Add 1/10 volume of 3M NaOAc pH 5.3
  3. Add 2 volumes 100% EtOH
  4. Either (1) collect spool of DNA with a pipette and wash in a new tube of 70% EtOH or (2) spin for 10 min. at full speed, pour off supernatent and wash pellet in 70% EtOH
  5. Pour or aspirate off EtOH and let pellet air dry.
  6. Resuspend pellet in water or TE.