Proteinase K Preparation
For 100ml of storage buffer:
| 50 ml |
Glycerol |
| 1 ml |
1M Tris-HCl pH 7.5 |
| 0.29g |
CaCl2 |
| ddH20 |
to 100ml |
For 10 ml of Proteinase K stock solution:
- Put 10ml of storage buffer in a 15ml screw-cap tube
- Add 100mg of Proteinase K
- mix well by inverting until crystals are dissolved
- put 1ml aliquots into 1.5ml microfuge tubes
Store at -20oC