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Analyzing BaseStation Sequencing Gels using Cartographer
Software from MJ Research 1.
Select gel by double-clicking. Designate
file path by clicking on button with three dots.
Select the dat file under the gel folder, then click Close.
File size should change. 2.
Select the gel again. Click
on the button with the three dots following analysis location.
Select “In Gel Directory”, then select “Don’t do anything
to existing files”. Click
button with three dots after gelpath.
Find the samples file under the gel directory.
Click OK. Click Close. 3.
Go to gel view. Track the
gel. Do not accept the
computer’s tracking and only interpolate about 6 lanes at a time. 4.
Extract the lanes. 5.
Go to analysis view. 6.
Go to Tools, then Trim & threshold basecalls. Only select “Change basecalls according to quality”.
The quality should be 20 and designation should be N.
Apply to all lanes. 7.
Examine all lanes. If lane is
bad (sequence failed, insufficient flanking region [less than 50 good
bases], no repeat), click “View Next Lane”.
If lane is good, click “Export & View Next”.
Save under the appropriate library as a “.scf” file. 8.
After examining all lanes, close gel.
Do not save gel analysis. 9. Rename the exported files to the sample name. |
Last Updated: 11/04/02
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