1 M Tris-Cl

Dissolve 121.1 g of tris base (sigma # T-1503) in 700 ml of ddH2O.  Bring to desired pH (Usually 7.5 or 8.0) with concentrated HCl.  Bring volume to 1L with ddH2O.  Filter with 0.5 micron filter.  Autoclave.  Store at room temperature.