10X TNE Buffer
(100mM Tris; 2.0M NaCl; 10mM EDTA; pH 7.4)
To 800ml ddH
2
O add:
Trizma Base
12.1g
T-1503
disodium EDTA
3.7g
E-5134
NaCl
116.8g
pH 7.4 with Conc. HCl
bring to volume of 1L with ddH
2
O.
Filter and autoclave
Store at 4 C